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KMID : 0545120200300050777
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Journal of Microbiology and Biotechnology
2020 Volume.30 No. 5 p.777 ~ p.784
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Characterization of Two Self-Sufficient Monooxygenases, CYP102A15 and CYP102A170, as Long-Chain Fatty Acid Hydroxylases
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Rimal Hemraj
Lee Woo-Haeng
Kim Ki-Hwa
Park Hyun
Oh Tae-Jin
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Abstract
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Self-sufficient P450s, due to their fused nature, are the most effective tools for electron transfer to activate C-H bonds. They catalyze the oxygenation of fatty acids at different omega positions. Here, two new, self-sufficient cytochrome P450s, named ‘CYP102A15 and CYP102A170,’ from polar Bacillus sp. PAMC 25034 and Paenibacillus sp. PAMC 22724, respectively, were cloned and expressed in E. coli. The genes are homologues of CYP102A1 from Bacillus megaterium. They catalyzed the hydroxylation of both saturated and unsaturated fatty acids ranging in length from C12?C20, with a moderately diverse profile compared to other members of the CYP102A subfamily. CYP102A15 exhibited the highest activity toward linoleic acid with Km 15.3 μM, and CYP102A170 showed higher activity toward myristic acid with Km 17.4 μM. CYP10A170 also hydroxylated the Eicosapentaenoic acid at ω-1 position only. Various kinetic parameters of both monooxygenases were also determined.
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KEYWORD
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Bacillus sp., cytochrome P450, fatty acid hydroxylation, Paenibacillus sp., self-sufficient monooxygenase
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